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cell signaling technology cat 18583s  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc cell signaling technology cat 18583s
    Cell Signaling Technology Cat 18583s, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 421 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+signaling+technology+cat+18583s/BRD4+Rabbit+mAb/pmc13003987-89-24-24
    Average 96 stars, based on 421 article reviews
    cell signaling technology cat 18583s - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Software:

    Article Title: SKP2 regulates TERT independently of RUNX1/ETO in AML subtypes lacking the t(8,21) translocation via CDKN1B and RB phosphorylation states
    Article Snippet: .. Following this image band image were obtained using the VersaDoc system (BIO-RAD) and quantification was carried out using the ImageLab software (BIO-RAD) Antibodies used in this experiment were; SKP2 (Rabbit polyclonal, Cell Signaling Technology, Cat# 4358), β-actin (Rabbit polyclonal, Cell Signaling Technology, Cat# 4967), E2F1 (Rabbit polyclonal, Cell Signaling Technology, Cat# 3742), CMYC Rabbit monoclonal, Cell Signaling Technology, Cat18583S), Underphosphorylated-RB (Mouse monoclonal, BD Pharmingen, Cat#554164), CDKN1B (Rabbit polyclonal, Cell Signaling Technology, Cat# 2552), FOXO3a (Rabbit polyclonal, Cell Signaling Technology, Cat# 2497), TERT (Rabbit Polyclonal, Merck milipore ABE2075). ..

    Article Title: SKP2 regulates TERT independently of RUNX1/ETO in AML subtypes lacking the t(8,21) translocation via CDKN1B and RB phosphorylation states.
    Article Snippet: .. Following this image band image were obtained using the VersaDoc system (BIORAD) and quantification was carried out using the ImageLab software (BIO-RAD) Antibodies used in this experiment were; SKP2 (Rabbit polyclonal, Cell Signaling Technology, Cat# 4358), β-actin (Rabbit polyclonal, Cell Signaling Technology, Cat# 4967), E2F1 (Rabbit polyclonal, Cell Signaling Technology, Cat# 3742), CMYC Rabbit monoclonal, Cell Signaling Technology, Cat18583S), Underphosphorylated-RB (Mouse monoclonal, BD Pharmingen, Cat#554164), CDKN1B (Rabbit polyclonal, Cell Signaling Technology, Cat# 2552), FOXO3a (Rabbit polyclonal, Cell Signaling Technology, Cat# 2497), TERT (Rabbit Polyclonal, Merck milipore ABE2075). ..

    Incubation:

    Article Title: The synergy of TPL and selinexor in MLL-R acute myeloid leukemia via Rap1/Raf/MEK pathway-mediated MYC downregulation.
    Article Snippet: MLL gene rearrangement recurrently occurs in acute myeloid leukemia (MLL-r AML), which is closely associated with chemotherapy insensitivity and unfavorable clinical outcomes.. More importantly, there are limited therapeutic options for the management of patients with MLL-r AML, thus necessitating novel effective treatment strategies.. In this study, we demonstrated that low doses of triptolide (LD TPL) and the XPO1 inhibitor selinexor exerted synergistic therapeutic effects on poor-outcome MLL-r AML in vitro, ex vivo and in vivo.

    Article Title: The synergy of TPL and selinexor in MLL-R acute myeloid leukemia via Rap1/Raf/MEK pathway-mediated MYC downregulation
    Article Snippet: Slides were processed for immunohistochemistry staining by Leica RM2245 (Leica Biosystems, Wetzlar, Germany). .. Tissue slides were immersed in boiling citrate buffer for 30 min for antigen retrieval and incubated with primary antibody against human PARP (Cell Signaling Technology, Cat# 9532s ), Caspase3 (Cell Signaling Technology, Cat #9662S), Cleaved-Caspase3 (Cell Signaling Technology, Cat# #9664S), c-Myc (Cell Signaling Technology, Cat18583S) overnight at 4 °C after blocking with 3 % BSA solution. ..

    Article Title: The Effect of AZD5153 on Radiosensitivity in Pancreatic Cancer Cells Through ATM-chk1 Pathway
    Article Snippet: .. The membranes were blocked with 5% skim milk for 2 h, and incubated with primary antibodies against BRD4 (Cell Signaling Technology, Cat# 13440s), c-Myc (Cell Signaling Technology, Cat# 18583s), p-ATM (Abcam, Cat# ab81292), ATM (Cell Signaling Technology, Cat# 2873s), p-cdc25C (Cell Signaling Technology, Cat# 4901s), cdc25C (Cell Signaling Technology, Cat# 4688s), p-chk1 (Cell Signaling Technology, Cat# 2348s), chk1 (Abcam, Cat# ab40866), p-cdc2 (Cell Signaling Technology, Cat# 4539s), cdc2 (Cell Signaling Technology, Cat# 9116s), γ-H2AX (Cell Signaling Technology, Cat# 9718s), cleaved PARP (Cell Signaling Technology, Cat# 5625s), Bax (Cell Signaling Technology, Cat# 14796s), β-actin (Abcam, Cat# ab6276),Vinculin (Cell Signaling Technology, Cat# 13901s) overnight at 4 °C. .. After washing with Tris buffered saline with Tween 20 (TBST) for 3 times, the membrane followed by incubation with the appropriate HRP-conjugated secondary antibody (Beyotime, Cat# A0208/Cat# A0216) for 1 h at room temperature.

    Article Title: The Effect of AZD5153 on Radiosensitivity in Pancreatic Cancer Cells Through ATM-chk1 Pathway
    Article Snippet: .. The membranes were blocked with 5% skim milk for 2 h, and incubated with primary antibodies against BRD4 (Cell Signaling Technology, Cat# 13440s), c-Myc (Cell Signaling Technology, Cat# 18583s), p-ATM (Abcam, Cat# ab81292), ATM (Cell Signaling Technology, Cat# 2873s), p-cdc25C (Cell Signaling Technology, Cat# 4901s), cdc25C (Cell Signaling Technology, Cat# 4688s), p-chk1 (Cell Signaling Technology, Cat# 2348s), chk1 (Abcam, Cat# ab40866), p-cdc2 (Cell Signaling Technology, Cat# 4539s), cdc2 (Cell Signaling Technology, Cat# 9116s), γ-H2AX (Cell Signaling Technology, Cat# 9718s), cleaved PARP (Cell Signaling Technology, Cat# 5625s), Bax (Cell Signaling Technology, Cat# 14796s), β-actin (Abcam, Cat# ab6276),Vinculin (Cell Signaling Technology, Cat# 13901s) overnight at 4 °C. .. After washing with Tris buffered saline with Tween 20 (TBST) for 3 times, the membrane followed by incubation with the appropriate HRP-conjugated secondary antibody (Beyotime, Cat# A0208/Cat# A0216) for 1 h at room temperature.

    Blocking Assay:

    Article Title: The synergy of TPL and selinexor in MLL-R acute myeloid leukemia via Rap1/Raf/MEK pathway-mediated MYC downregulation.
    Article Snippet: MLL gene rearrangement recurrently occurs in acute myeloid leukemia (MLL-r AML), which is closely associated with chemotherapy insensitivity and unfavorable clinical outcomes.. More importantly, there are limited therapeutic options for the management of patients with MLL-r AML, thus necessitating novel effective treatment strategies.. In this study, we demonstrated that low doses of triptolide (LD TPL) and the XPO1 inhibitor selinexor exerted synergistic therapeutic effects on poor-outcome MLL-r AML in vitro, ex vivo and in vivo.

    Article Title: The synergy of TPL and selinexor in MLL-R acute myeloid leukemia via Rap1/Raf/MEK pathway-mediated MYC downregulation
    Article Snippet: Slides were processed for immunohistochemistry staining by Leica RM2245 (Leica Biosystems, Wetzlar, Germany). .. Tissue slides were immersed in boiling citrate buffer for 30 min for antigen retrieval and incubated with primary antibody against human PARP (Cell Signaling Technology, Cat# 9532s ), Caspase3 (Cell Signaling Technology, Cat #9662S), Cleaved-Caspase3 (Cell Signaling Technology, Cat# #9664S), c-Myc (Cell Signaling Technology, Cat18583S) overnight at 4 °C after blocking with 3 % BSA solution. ..



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